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Next, the stable transduction frequencies (STFs) reached by dHV.F50 and by dHV.68/5'3'.F50 were determined by extensively subculturing both types of dividing cells and by measuring the percentages of eGFP-positive cells through flow cytometry as a function of time.
Cell cycle disruption by gefitinib was investigated by flow cytometry as a function of both the drug concentration and length of incubation.
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The expression of Foxp3 and CD25 was assessed by flow cytometry as a marker of conversion.
Confocal microscopy provided further evidence corroborating the cytotoxicity results from MTT assays and flow cytometry, as well as the co-staining of PE-Annexin V and T-1 in flow cytometry analyses.
The use of flow cytometry as a tool for monitoring filament formation of fungi.
The CD8+ V-gene usage in flow cytometry and TCRB-sequencing correlated well (Fig. 1b; P<0.0001, Spearman correlation coefficient r=0.61), thus justifying the use of flow cytometry as a screening and sorting method.
Flow cytometry data were plotted as a function of fluorescence intensity FL-1 (green) versus FL-3 (red) fluorescence.
Again, CXCR3 expression was analysed by flow cytometry as read-out of the experiments.
CD8+ cytotoxic function was analyzed using flow cytometry as described previously.
Cell cycle analysis and detection of apoptosis were performed using flow cytometry as described previously11.
Phases of cell cycle were analyzed using flow cytometry as described previously [59].
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