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At last, the experimental results were detected using Annexin-V method on a Beckman Coulter Epics XL MCL flow cytometer system (BD Accuri C6), and the percentage of apoptotic cells was clarified by software analysis (Flowjo 7.6.2).
Cells were analyzed by the Guava easyCyt flow cytometer system.
Cells were acquired with the use of the Accuri 6 flow cytometer system.
The Rho 123 accumulation in cells was determined by the Guava EasyCyt flow cytometer system.
Flow cytometry analysis was performed by LSR II Flow Cytometer System (BD Biosciences).
Samples were analyzed on an Accuri C6 flow cytometer system (Accuri Cytometers, Ann Arbor, MI) as described [ 33].
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The kit is designed for use with the Amnis ImageStreamX Mark II and the Amnis FlowSight imaging flow cytometers, systems that combine the quantitative power of flow cytometry with the spatial information provided by microscopy.
The samples were then analyzed directly from the 96 well plate using a C6 Flow Cytometer® System ™ (Accuri cytometers) in a high throughput format.
Accuri's C6 Flow Cytometer™ System (Accuri Cytometers, Ann Arbor, MI) was used for analysis.
Cell viability was determined by measuring the fluorescence emission at 530 nm with a flow cytometer (FACScan System, Becton Dickinson Instruments, USA).
The ROS level was determined by measuring the fluorescence emission at 530 nm with a flow cytometer (FACScan system, Becton Dickinson Instruments, USA).
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