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We adopted this approach using a nebulizer with a very small dead volume (< 0.1 mL) and a computer controlled mass flow controller.
Also, the V/III flow rate was controlled by mass flow controller.
In the elution mode the cross-flow is measured and controlled with a flow controller device.
The flow rate of oxygen gas was controlled by a mass flow controller.
The purity of argon gas in this work was 99.998% and controlled by a mass flow controller.
The airflow rate was controlled with a mass flow controller.
The flow rate and type were controlled by a programmable flow controller device (Osci-Flow, FlexCell).
Gas flow rate was 200 ml min-1 of house air and controlled using a mass flow controller (Cole-Parmer Instrument Company, IL, USA).
Anaerobic conditions were maintained by sparging the culture broth with 0.5 L/min nitrogen gas (Grade 5.0, containing less than 3 ppm oxygen; PanGas AG, Switzerland) controlled by a gas flow controller (Get red-y, Vögtlin Intruments AG, Switzerland).
The levitation gas was regulated with a computer-controlled VÖGTLIN red-y mass flow controller (0 - 1000 mL min-1).
The speed of airflow was controlled by a GFC mass flow controller (Aalborg, Orangeburg, NY).
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