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To identify potential candidate genes, we compared the detected QTLs with the scaffold assembly and annotation of the Japanese flounder reference genome.
We then used the Japanese flounder reference gene set generated from the PoGSP database along with medaka (Oryzias latipes) and zebrafish (Danio rerio) gene sequences from Ensembl (release 57).
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Another NMR metabolomics study involving flounder sampled from industrialized and reference sites in the United Kingdom showed that water composition had a significant effect on the fish liver metabolome (Parsons et al. 2007).
Flounder liver extracts from contaminated and reference sites were analyzed, and multivariate statistical analyses confirmed high reproducibility across all seven laboratories involved in the study.
Random samples of the flounder populations from the indirectly polluted and reference mesocosms were examined every 2 months for epidermal diseases (lymphocystis, skin ulcers, fin rot) and then released.
Another example of the utility of micro-array analysis of fish from polluted areas is a study comparing transcripts in male flounder collected from an affected site (Tyne) and a reference site (Alde) in the United Kingdom (Williams et al. 2003).
Part of that punch is a car topped by a fish tomato sculpture, nicknamed Fishy Tomato, a reference to the much-vilified DNA Plant Technology hybrid that mixed a tomato with genetic material from the Arctic flounder.
A flounder?
Long Island, it's flounder.
A talking flounder?
Snapper, bluefish, blowfish, flounder.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com