For disease severity test, four florets of two spikelets in the middle of head were inoculated per each genotype.
In the transcriptomics experiment 12 florets per head (the two basal florets of six central spikelets) were inoculated at anthesis with 10 μL of a F. graminearum conidia spore suspension (strain IFA65, concentration 50,000 conidia/mL) or mock by cautiously inserting a droplet onto the generative part of each floret.
Lynx, floret tissue of six wheat heads per genotype, treatment and sampling timepoint were pooled prior to RNA extraction in order to reduce the biological variation between the samples.
I hate to spoil the naughtiness of faux bread heaven for you, but cauliflower is low in fat and carbs, high in fiber and vitamin C -- three florets of cauliflower a day provide 67percentt of one's daily vitamin C requirement.
We examined four panicle traits number of florets per panicle (FPP), number of primary branches per panicle (BPP), number of florets per primary branch (FPB), and frequency of pre-anthesis floret abortion (FA)—of four cultivars (Takanari, Akihikari, Lemont, IRAT109).
At the heading stage, pollen grains of the top five florets of primary panicles were mixed and stained with 1% I2-KI solution.
Anthers were recovered from upper florets with two pairs of sharp forceps, measured with a micrometer, and collected directly into screw-cap microcentrifuge tubes chilled in liquid nitrogen.
The sorghum florets contain two spikelets, one of which contains both the anthers and the ovary, whereas the other is often sterile.
All florets of the strong phenotype plants and approximately half the florets of the moderate phenotype plants had less than the six stamens found in wild-type (Table 2).
