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Floating sections were processed for immunostaining as described previously [7].
Freely floating sections were cut at 30 µM in a cryostat.
Floating sections were incubated overnight at room temperature (RT).
All incubations were performed on floating sections at room temperature (unless otherwise stated), under gentle rocking.
Floating sections (30 μmol/L thickness) were obtained using a sliding microtome.
Floating sections were used to detect location of electrode, cholinergic cells, and neurogenesis.
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Fixed free-floating sections were washed with PBS and treated with 3% hydrogen peroxide solution.
c-fos protein expression was characterized in free-floating sections according to the following protocol.
Free-floating sections were processed using a standard immunohistochemical procedure.
All the reactions were performed on free-floating sections.
Stainings were performed on free-floating sections under continuous agitation.
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