Sentence examples for floating culture from inspiring English sources

The co-cultured collagen gels were subjected to floating culture for an additional 5 days, followed by hematoxylin and eosin staining.

After 6 days as a floating culture, EBs were transferred to gelatin-coated plate and cultured in the same medium for another 10 days.

Furthermore, to evaluate the cell viability of these two populations under floating culture conditions, the PANC-1 cells of these two populations cultured under 6-day floating conditions were reseeded at a density of 1000 cells/well onto six-well attachment plates supplemented with DMEM containing 10% FBS.

Floating culture efficiently induced human ADMSCs into functional HLCs in vitro (Okura et al., 2010).

Cells were treated with Lefty-A and Dkk-1 during floating culture.

To analyze the differentiation potential of these rat iPS cells in vitro, we used floating culture to induce EBs.

These aggregates were then seeded into microchambers coated with fibronectin and bovine serum albumin (BSA) to establish adherent and floating cultures, respectively, both of which are frequently used to differentiate hiPSCs.

For this purpose, buffalo ES cells were differentiated as embryoid bodies (EB) in floating cultures and as monolayer adherent cultures in different doses (10%, 20%, and 40%) of TCM for different culture intervals (4, 8, and 14 days), to identify the optimum dose-and-time period.

Continuous, non-transformed NSC lines have been established from the developing human (h) brain, either prospectively [7] or by epigenetic stimulation in floating cultures [8], [9], [10] and in adherent monolayers [11].

For RNA extraction S. macrospora was grown for 3, 4, 5, 6 and 7 days in liquid BMM medium at 27°C in floating cultures to induce sexual reproduction conditions and in Erlenmeyer flasks with 100 ml of liquid BMM medium shaken at 130 rpm to induce vegetative development as described before (Nowrousian and Cebula 2005).

ACCS cells were seeded at a density of 5 × 10 cells/mL in 60-mm noncoated dishes with serum-free DMEM containing 40 ng/mL basic fibroblast growth factor (bFGF) and 20 ng/mL epidermal growth factor (EGF) for floating cultures.