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Care was taken to include both adherent and floating cells, although few floating cells were observed.
Following incubation medium and the floating cells were gently and completely removed using a micropipette.
After 1 hr, floating cells were removed.
Floating cells were collected and plated in T75 flasks.
Both attached and floating cells were counted.
Additionally, floating cells could not be observed either.
Adherent and floating cells were combined for subsequent procedures.
Some of the floating cells were found to be swollen.
Media was collected and spun down to collect floating cells.
Culture medium containing floating cells was removed and retained.
The adherent and floating cells were harvested and counted.
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