Sentence examples for floating aggregate from inspiring English sources

Figure 6d shows beads falling from the floating aggregate immediately after its formation.

In a serum-free and feeder-free floating aggregate culture, Osakada et al 58 found that ESCs and iPSCs could be efficiently differentiated into RPE cells by supplementing CKI-7 (a Wnt antagonist) and SB-431542 (a nodal antagonist).

To start this culture, a fixed number of dissociated ESCs (e.g. 3,000 cells for mouse ESCs) are reaggregated in each well of a 96-well plate that has a special surface coating for blocking cell-plate adhesion (Fig. 2A), and are cultured as a floating aggregate in serum-free medium.

Floating aggregate culture of ESCs has the advantage of mimicking tissue formation in a three-dimensional (3D) context, and an efficient method for rostral forebrain differentiation is SFEBq culture (serum-free floating culture of embryoid body-like aggregates with quick reaggregation) [ 25, 27, 28].

The beads formed the single floating aggregate of Figure 6a immediately water was added, but they disaggregated and sank with time.

Notably, we found that floating aggregates eventually adhered to surfaces coated with BSA upon differentiation, and that differentiation depends on the initial size of aggregates.

Medium was replaced or supplemented with fresh growth factors twice a week until cells started to grow and form floating aggregates.

During their evolution, primary cultures were always comprised of colonies with varying morphologies (floating aggregates that exclude trypan blue, giant cell colonies, fibroblastoid and cobblestoned clusters), despite being in the same flask with an apparently identical TME (Figure 4A).

Briefly, clusters of hESCs were cultured as floating aggregates in defined medium, supplemented with the bone morphogenetic protein antagonist, noggin, basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF).

In the present work, using the same experimental conditions, we were able to derive floating aggregates of NS cells from all stages of the in vitro neuroepithelial rosette cultures (days 4, 8, 12, 16 and 20) with similar efficiencies (Fig. 4A,B).

For suspension cultures or cells that grow as floating aggregates, 2-ml plastic freezing vials are suitable containers for transport.