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The cell lysate was overlaid with a 30 5% sucrose step gradient, and separated for 16 h at 38 000 r.p.m. by isopycnic floatation in a Beckman SW-41 rotor. 1 ml fractions were collected and sedimented in a Ti70.1 rotor at 40 000 r.p.m. for 1 h.
After 12 hours, the larvae were collected by floatation in a 20% glucose solution, rinsed with phosphate buffered saline (PBS), and transferred again, either to a fresh plate with glucose fly food or to a fresh plate with fly food containing 555 mM glucose plus 225 mM galactose.
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However, dry substrates are prone to floatation in an anaerobic digester [14] and hence poor contact with the microbial consortium.
The meiofauna fraction of all samples was extracted via decantation and floatation in Ludox using a 45 μm sieve according to the methods of Somerfield et al. [ 46].
After breaking from his mentally unstable brother Chuck Michael McKeann), who never wanted Jimmy to succeed, he's given up the lawyering life and is pulling petty cons while floating in a lazy river at a New Mexico resort, with his drink in his floatation device, his snacks on a raft by his side, and his cellphone floating in a plastic bag.
The FOGs are located in the fatty waste that is collected by floatation in grease traps.
Royal Mail said that, as a result, it was more than halving its expected growth rate in the UK parcel market: the 6 per cent it predicted at its floatation in 2013 was chopped to 1 per cent to 2 per cent for the next two years.
According to the properties of LNG, this work aims to develop a direct contact type ice generator, which integrating some special designs such as jacketed cylinder, nozzle arrangement and gas floatation, in order to improve heat transfer and phase separation in the SFD process.
Then he heads to Isolation Floatation in Colorado to learn more.
The method used to fractionate MBM into soft tissue and bone particles involves floatation in chloroform, which has the side effect of partially defatting the particles.
We thus prepared post-nuclear supernatants (PNS) from mock-treated or p11 siRNA2-treated HeLa cells (Fig 5C left panel), which were then further fractionated by floatation in gradients to purify membrane fractions containing both early and late endosomes (Fig 5C right panel), [24].
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