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Flies of all strains were kept at 18°C until being tested in 2012 and 2013.
Female virgin flies of all species were collected upon eclosion and kept in groups of up to 10 flies from a single-species.
Flies of all species were reared on standard resin-sugar-yeast-agar medium containing proprionic acid and methylparaben as mold inhibitors.
To confirm whether the Hsp70 genes were deleted from the Hsp70- strain, we extracted DNA from individual flies of all strains according to Gloor et al [ 47].
The developmental delay of tko 25t mutant flies was slightly greater in males than in females, as observed previously (Kemppainen et al. 2009), and an additional delay of approximately 1 d was produced in flies of all genotypes and both sexes by the presence of RU486 in the food.
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We found that between10%% (thorax) and 23% (whole fly) of all metabolites were affected by DR at one or more of the three sample ages (10, 20, and 40 days).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com