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We addressed this issue by showing that all flies in this study were infected with Wolbachia.
Cpr97Eb gene is strongly expressed during pupal wing morphogenesis [29], and the morphological defect of the wing disc-restricted RNAi flies in this study suggests that Cpr97Eb protein is involved in the TG-dependent crosslinking required for cuticle morphogenesis and sclerotization.
In the case of immune responsive genes, the same trend, observed on 40 day old flies in this study, can be also observed for 61 day old male flies according to the data of Landis et al.. Age related impairment in immunity has been reported recently [ 45], which results in a prolonged response following immune challenge.
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The infection prevalence of 9.4% in submitted flying foxes in this study is not statistically significantly higher than that previously observed (6%) in sick, injured, and orphan flying fox submissions (3; H.E. Field, unpub.data); the wide 95% confidence interval (CI) (4% to 18%) reflects the limited sample size in this study.
Both the CRUK flyer in this study and the materials in the study by Hewitson et al. were sent together with NHS gFOBT kits, suggesting that although Cancer Research UK is a well known charity, it could be that a letter from someone's own GP provides a stronger endorsement.
Flies used in this study include Wolbachia-infected and uninfected Drosophila simulans Riverside flies (DSR and DSRT, respectively) and D. melanogaster Canton (DMC and DMCT, respectively).
The different UAS-dsRNA flies used in this study are from the VDRC (Vienna, Austria: GD18551, KK105942), and the Fly Stocks from the National Institute of Genetics NIGG, Kyoto, Japan: 7280 R-1).
All the flies used in this study were kept and tested at room temperature (21 22°C) unless described otherwise.
Transgenic flies used in this study were UAS-hig-HA, hs-Appl-FLAG, UAS-Appl-HA and UAS-yata-HA.
Flies used in this study were derived from a wild population of D. melanogaster collected in Terhune, New Jersey in 1999 by Valerie Pierce (NJ population).
All the transgenic flies used in this study were constructed with a GMR-Gal4 driver to ensure strong and specific expression in the retina throughout postembryonic stages [21].
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