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However, spin radical labeling at two flexible sites located in different secondary structure elements has not been reported yet.
In order to investigate the effect of viscosity on distance measurements between two flexible sites, MTSL labeling was performed on T4 lysozyme (T4L).
A strategy has been proven effective in enhancing proteins' thermostability with two steps: predict flexible sites of proteins firstly and then rigidify these sites.
We refer to this approach as rigidify flexible sites (RFS) and give an overview of such a method through summarizing the methods to predict flexibility of a protein, the methods to rigidify residues with high flexibility and successful cases regarding enhancing thermostability of proteins using RFS.
In this study, we used a protein engineering approach; rigidify flexible sites, to improve stability of cABC I. B-factor analysis showed a flexible loop at the N-terminal domain of cABC I which may be involved in its thermal instability and five residues in this region were replaced with proline.
They are structurally flexible sites.
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Furthermore, the total dinucleotide-based stiffness parameter ktotal (see Methods for definition) unveiled that LRs (in both naked and nucleosomal DNA) are located in regions with large variations in flexibility, where an extremely flexible site is surrounded by stiff motifs.
E.P.R.I. sees "technical merit" in a more flexible, site specific approach.
Small high temperature gas-cooled reactors (HTRs) have the advantages of transportability, modular construction and flexible site selection.
Moreover, small HTRs have advantages over large nuclear reactors of demonstrated inherent safety, transportability, modular construction, and flexible site selection.
At the local scale, a flexible, site specific leaching model that can be adjusted to the actual data availability is used to estimate the mass flux over time from identified sites.
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