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In addition, the flexible loop region between the including residues 78 81 has full torsional freedom.
In the S174A mutant, the lack of phosphorylation at S174 causes the C-terminal helix to become a flexible loop.
The flexible loop regions were modeled using high temperature simulated annealing and constrained molecular dynamic simulations.
Most apparent among these are the ability to tetramerize and the presence of a flexible loop.
According to [24], Cys139 should be rather located near the flexible loop (Figure 5).
However, partly denatured antigen induced by Freund's adjuvant could increase the response to flexible loop regions in experimental immunizations [34].
Thus, if GSH simply ligates with Glo1, the flexible loop closes and prevents other substrates from entering.
Interestingly, position 158 is located in the flexible loop which connects the pilin and the lectin domains (Figure 6B).
Thus, the competitive peptide should prevent the flexible loop region of endogenous YB-1 from binding to RSK.
Now the flexible loop is expected to drift into cluster cl1, protruding more into the SUB and opening gate 1.
In step 2 the COF should already be occluded and gate 1 being closed by the motion of the flexible loop into cl3.
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