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The inclusiveness of the array design allowed a flexible gene expression analysis, initially targeting high confidence transcripts and then extending to a more speculative set.
In this study, we predicted that environmentally flexible gene expression in the branching coral Acropora millepora would be associated with signatures of weak gene body methylation.
In non-malignant cells, epigenetic mechanisms are used to ensure flexible gene expression during development but later also permanent silencing of genes in differentiated tissues.
Roberts and Gavery [ 11] proposed a role for gene body methylation in modulating plasticity in which weak gene body methylation passively facilitates flexible gene expression via alternative transcription start sites (TSSs), increased sequence mutations, exon skipping, or through transient gene methylation.
We have developed and validated a new and flexible gene expression profiling tool, in the form of a 15 K oligonucleotide DNA microarray, which produced biologically relevant information agreeing with prior observations made in the literature, providing external validation for our new resource.
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A graduate of MIT and UC Berkeley, her research uses computational and molecular biology to understand how post-transcriptional regulation leads to robust and flexible control of gene expression.
Secondly, the lengths of many of the cells of the CNS means that controlling the rates of translation of localized mRNA pools will likely offer faster and more flexible control of gene expression than changes in the levels of transcription, which would necessarily involve transport of either newly synthesized mRNA or protein.
In comparison to the Affymetrix® platform, evaluated platforms enable higher sample throughput, having shorter turnaround time for results and provides more flexible solutions for focused gene expression of a specific sets of genes.
Linear models have proven to be a powerful and flexible approach for analysis of gene expression experiments (Smyth, 2005).
While conventional identification of splice variants generally targets individual genes, we present here a new exon-centric array (GeneChip Human Exon 1.0 ST) that allows genome-wide identification of differential splice variation, and concurrently provides a flexible and inclusive analysis of gene expression.
In contrast, sequencing and gene expression profiling are flexible technologies allowing individual conditions or cellular states to be independently sampled.
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