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The polymer molecule bending resulting from this association can contribute to an enhanced PBO chain flexibility in solution.
Moreover, the full-length PLA2 demonstrates increased conformational flexibility in solution and is stabilized upon membrane binding, while the ΔN10 fragment is more rigid than the full-length PLA2 both in free and membrane-bound states.
Also, the helical structures are not rigid as observed in protein crystal structures and show considerable conformational flexibility in solution.
Electron density for the 23-residue aldehyde substrate-binding loop is absent, implying substantial conformational flexibility in solution.
NMR and all-atom MD are now the methods of choice for investigation of protein flexibility in solution.
Pn14 exhibited greater flexibility in solution, and thus paid a greater entropic penalty upon binding to the antibody, as compared to GBSIII.
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Recommendations are: The need for a sense of urgency, powerful management, flexibility in solutions, collaborative design, risk acceptance and process management.
This parallel between the sequence-dependent DNA flexibilities in solution (TRX scores) and the minor groove widths in X-ray structures strongly supports the notion that this relation is a general intrinsic property of DNA.
However, were this group to retain the flexibility observed when unbound upon binding (i.e., oscillation between two modes; see Figs. 2C and 5), the electron density map could be effectively accounted for; it is therefore proposed that the X-ray data is better accounted for by a structural model in which the flexibility observed in solution for this group is retained on binding.
Molecular modeling starting from 3000 randomized TT30 conformations showed that high-quality X-ray curve fits were obtained with extended SCR arrangements, showing that TT30 has a limited degree of inter-SCR flexibility in its solution structure.
This figure indicates that a = 0.6 is a good compromise for this dataset because it allows flexibility in the solution while the factor spectra still show high similarity to reference profiles.
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CEO of Professional Science Editing for Scientists @ prosciediting.com