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To assess the quality of 454 Flex sequence assembly, we selected two BACs SC172L01 and SC118L15 for Sanger sequencing.
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Full length BAC sequences were generated with Roche 454 Flex sequencing also at NRC.
SM, FN, and JW carried out 454 Flex sequencing and BAC sequence assembly.
The LAVA-Flex sequence was used as a reference.
On 23 (92%) of the images of the LAVA-Flex sequence, the artifacts by the dental alloys showed multiple hyperintense rings (Figure 3).
A retrospective study was undertaken on 10 patients with sequentially performed PET/CT and MR. The LAVA-Flex sequence was investigated, with in-phase (IP) and out-phase (OP) echoes.
Subgroup analysis for patients with low to moderate (1 to 3) blurring artifacts on LAVA-Flex sequence (group 1) and patients with extensive to non-diagnostic blurring (group 2) was performed.
The largest artifact size of 612 mm2 on axial images was observed on LAVA-Flex sequences and could be reduced to 195 mm2 for T1-FSE sequence to 131 mm2 for MAVRIC SL and to 126 mm2 for MAVRIC-fast (Table 1).
Based on the assessment of spatial blurring on LAVA-Flex sequences, two groups were generated: group 1 with low to moderate artifacts (categories 1 to 3) and group 2 with blurring artifacts impairing diagnosis (categories 4 and 5).
The absolute reduction of signal void from LAVA-Flex sequences to the T1-weighted fast spin-echo (FSE) sequences was 416 mm2 (range 4 to 2,010 mm2) to MAVRIC 481 mm2 (range 12 to 2,288 mm2) and to MAVRIC-fast 486 mm2 (range 39 to 2,209 mm2,209
An axial two-point Dixon-based T1-weighted 3D gradient echo sequence (LAVA flex) was acquired and fat-suppressed images were reconstructed for all stations.
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