Sentence examples for flex peroxidase from inspiring English sources

Briefly, deparaffinized and rehydrated sections were immersed in Tris-buffered saline-Tween 20 (TBS-T) and endogenous peroxidase blocked by Envision Flex Peroxidase Blocking Reagent (Dako) for 10 minutes.

A standardised Dako EnVision FLEX Peroxidase Blocking reagent (K800, DAKO) and mouse monoclonal antibodies for CD68 (IS609, DAKO), CD163 (dilution 1 : 100, Cell Marque), and rabbit polyconal CD11b (dilution 1 : 100, Novus Biologicals, Littleton, CO, USA) were used for immunostaining following incubation for 20 min at room temperature.

Following that, staining was conducted with standardised Dako EnVision FLEX Peroxidase Blocking reagent (K800, DAKO) and polyclonal antibodies for CD3, CD8, CD4 (dilution 1 : 50; Dako) and FOXP3 (dilution 1 : 100: Sigma, Munich, Germany) following incubation for 120 min at room temperature.

Endogenous peroxidase activity and non-specific binding sites were blocked with EnVision FLEX Peroxidase-Blocking Reagent (Dako) for 5 minutes.

Endogenous peroxidase was blocked with EnVision FLEX Peroxidase-Blocking Reagent (Dako) before incubating the sections with respective monoclonal antibodies.

Endogenous peroxidase was blocked with EnVision FLEX Peroxidase-Blocking Reagent (Dako) before incubating the sections with respective monoclonal antibodies, which were produced by our group.

Endogenous peroxidase activity was blocked with EnVision FLEX Peroxidase-Blocking Reagent, before incubation with mouse monoclonal anti-human B7-H3 antibody (Clone MIH42, LifeSpan BioSciences, Seattle, WA), at 1 300 for 30 minutes at room temperature.

The protocol was as follows: slides were incubated for 5 min in an endogenous block (EnVision FLEX peroxidase-blocking reagent; Dako) and then incubated with antibody for 30 min.

Tendon sections were then washed in TBS/0.05% Tween buffer followed by a 5 min incubation with EnVision FLEX Peroxidase-Blocking Reagent to block the activity of endogenous peroxidase.

After removal of endogenous peroxidase (Flex Dako, Ready to Use, Dako) and rinsing in PBS, sections were incubated with polyclonal anti-CB1R receptor antibody (Abcam cat no.

After rinsing in Phosphate Buffer Saline, the slides were incubated with a biotinylated secondary antibody bound to a streptavidin peroxidase conjugate (Envision Flex kit Ref: K800021-2, Dako, Trappes, France).