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Peel and flesh tissues were sampled separately to determine how the position of the fruit on the tree might affect the levels of the primary and secondary metabolites in the fruit.
RNA samples from flesh tissue of watermelon fruits at the green, pink and red stage, as well as from leaf were provided to NimbleGen Systems , Inc
Tissue samples (gills or part of the fruitbody flesh) were stored in a CTAB 2X Buffer (100 mM Tris-HCl (pH 8), 1.4 M NaCl, 20 mM Na2 EDTA, 2% CTAB) until DNA extraction.
It is important to note that only generic peel and flesh tissue was evaluated so that buds for example were not independently sampled for PHs.
For PCR-based marker development and testing, leaf tissue samples from a subset of 28 individuals from the A194 population that showed reproducible flesh colour were collected and extracted following the protocol described by Gardiner et al. [ 45].
Tissue samples.
N.K. prepared the tissue samples.
Tissue samples were not blinded.
No autopsies were performed, no tissue samples taken.
L.P. provided the okapi tissue samples.
Under the agreement, hundreds of thousands of those tissue samples will go into a tissue bank for genetic studies.
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