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Fruit flesh samples were immediately frozen in liquid nitrogen and kept in −80 °C until use.
Flesh samples were taken by cutting two wedges, each approximately one-eighth of the fruit.
For all further analyses, flesh samples were taken from the heart area of each watermelon.
Center fruit flesh samples were collected at stages of 10, 18, 28, and 34 DAP, respectively.
De-boned and skinned flesh samples were combined into 3 pools per family for lipid analysis.
Soluble sugars were sampled using HPLC applying the procedure outlined at [ 62]; ethylene was sampled using GC-flame ionization detector (FID); pH of the flesh samples were measured by pH meter (PH-03 II), PH-03 IIument Corp., China) in juice squeeZD Instrument Corp.
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Approximately 10 ug cDNA from each of the four fruit flesh samples was used for sequencing on a Roche/454 GS-FLX Titanium instrument.
Older laboratory techniques to identify fish meat looked at the mix of proteins in flesh samples, but were unreliable, expensive and cumbersome.
High performance liquid chromatography (HPLC) analyses of allicin and alliin in garlic flesh and skin extract samples were performed by Silliker, JR laboratories, Burnaby, BC, Canada.
Whole fruits were peeled to a thickness of approximately 1 mm, and the remaining flesh was cut into pieces before all samples were flash-frozen in liquid nitrogen.
The samples were labeled as DP (purple-flesh tuber) and DW (white-flesh tuber), then immediately frozen in liquid nitrogen, and stored at −80°C prior to use.
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