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Approximately 10 ug cDNA from each of the four fruit flesh samples was used for sequencing on a Roche/454 GS-FLX Titanium instrument.
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When random samples are used, there is always potential error that comes with using a sample.
Soluble sugars were sampled using HPLC applying the procedure outlined at [ 62]; ethylene was sampled using GC-flame ionization detector (FID); pH of the flesh samples were measured by pH meter (PH-03 II), PH-03 IIument Corp., China) in juice squeeZD Instrument Corp.
Fruit flesh samples were immediately frozen in liquid nitrogen and kept in −80 °C until use.
Flesh samples were taken by cutting two wedges, each approximately one-eighth of the fruit.
For all further analyses, flesh samples were taken from the heart area of each watermelon.
Center fruit flesh samples were collected at stages of 10, 18, 28, and 34 DAP, respectively.
De-boned and skinned flesh samples were combined into 3 pools per family for lipid analysis.
For each sample (5% ethanol treated and control), approximately 10 g of mixed flesh (10 individuals) was used for RNA preparation and tannin concentration measurements.
Homogenized fish flesh was used for calibration.
About 10 g of homogenized flesh was used for the determination of titratable acidity and acidic profile.
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