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Total RNA was extracted from watermelon fruit flesh samples using the TRIzol Reagent (Invitrogen, USA).
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Approximately 10 ug cDNA from each of the four fruit flesh samples was used for sequencing on a Roche/454 GS-FLX Titanium instrument.
Total RNA was extracted from 1 g of flesh using the hot borate method [ 60].
A second 2003 sample was generated using the mesocarp tissue (berry flesh without skin).
Soluble sugars were sampled using HPLC applying the procedure outlined at [ 62]; ethylene was sampled using GC-flame ionization detector (FID); pH of the flesh samples were measured by pH meter (PH-03 II), PH-03 IIument Corp., China) in juice squeeZD Instrument Corp.
He provided a little help last month as scientists collected flesh samples from the animals for testing.
Comparing the three reconstructions for the orange flesh sample, the TV regularization shows the best reconstruction quality.
Using the flesh, squeeze out the juice and place in the bottom of the double boiler.
They also determined the literacy level of the tools used in these the studies using the Flesh-Kincaid methodology.
In Fig. 7, we present the reconstruction results obtained using the three methods for an orange flesh and a pearl sample.
For all further analyses, flesh samples were taken from the heart area of each watermelon.
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