The in vitro testing was accomplished by seeding MIA PaCa-2 cells into 96 well (Falcon 3072, Franklin Lakes, NJ, USA) flat bottom proliferation plates (5000 cells well−1).
None of the nanofibrous materials were cytotoxic compared to 2D (e.g., flat surface) controls; however, proliferation generally was inhibited on the nanofibrous scaffolds although to a lesser extent on the polysaccharide-derived materials compared to PES.
The PNM cells were separated from lymphocytes in similar way and stimulated with LPS for inducing their proliferation in flat bottom 96-well polystyrene microtiter and their proliferation was measured by tritiated thymidine (3H-Td) uptake method.
Carcinoma in situ (CIS) is characterized by flat, non-papillary, disordered proliferation and differentiation of urothelial cells.
Similar observations were reported by Garcia-Gareta et al., showing that nano-sized CaP crystals and relatively flat topographies enhanced MSCs proliferation, while rougher, microscale topographies enhanced osteogenic differentiation of MSCs.
This implies either that cell proliferation on flat film has a longer lag phase than when cultured on the honeycomb films, or that this DNA value represents a maximum number of cells sustainable by the 14 mm diameter discs.
The cells were cultured (37°C, 5% CO2) in duplicates in 24-well (for culture supernatant), or in triplicates in 96-well (for proliferation assay), flat-bottom tissue culture plates.
Osteoblasts grown on a fibrous matrix composed of multiwalled carbon nanofibers (100-nm in diameter) exhibit increased proliferation compared to those on flat glass surfaces[14 16].
Proliferation was very rare in flat cells (Table 3, Figure 4D and P).
Upon initiation of leaf development, adaxial-abaxial patterning is crucial for appropriate lateral expansion, via cellular proliferation, and the formation of flat symmetric leaves.
