If the bridge took a direct hit and the helm was knocked out, the men in the tiller flat would manually turn huge wooden wheels attached to the rudder to steer the ship.
Quantification of Venus-LMNA, CCND1-Venus and INCENP-GFP was performed manually on flat field and background corrected images using Fiji44.
The advantages are huge: I can cycle along manually on flat roads or the towpath, and when I come to a hill, of which there are many in Stroud, I can use power assist.
Flight ability was scored by placing the pup on a flat surface and either manually prodding it to fly or by holding the pup upside down by its feet and prodding it to fly.
The flat upper PDMS surface was manually aligned parallel to the cantilever.
The garbage receives mixed organic and inorganic domestic wastes, upon arrival to the composting site; the wastes were spread flat on the ground and sorted manually into organic and non-organic fractions.
At 2.5, 5, 6.25, 8, and 15 min after acidification, 3 μl of the acidified mixture was transferred onto plasma-cleaned, C-flat holey carbon grids (Protochips Inc ., manually blotted, and plunge frozen in liquid ethane.
In order to create fresh surfaces under ambient conditions, samples of magnesium alloy AM50 (Rocholl GmbH, Aglasterhausen, Germany) were cut and manually polished using dry and flat SiC sandpaper with a grit size of 800 mesh.
DOI: http://dx.doi.org/10.7554/eLife.06119.007 A total of 2100 F1Fo ATP synthase particles from the flattest regions of rectangular crystalline vesicles were manually selected and averaged by gold standard procedures (Scheres and Chen, 2012).
For primary screening, frozen flies were manually transferred to a new flat-bottomed 96-well plate (Daslab, Barcelona, Spain) by inverting the deep-well plate over this new plate.
