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They made whimsical still-life arrangements, filling laboratory flasks with colored water and making feather-duster bouquets.
Uninoculated flasks with the fortified medium served as the control.
Respective uninoculated minimal media flasks with heavy crude oil were also incubated as negative controls.
Data points represent mean values of three parallel culture flasks with standard deviations.
Values given are means of three parallel flasks with standard deviations.
The cells were seeded in T25 flasks with 52,000 cells per flask (~2100 cells/cm2).
The samples were stirred continuously in closed flasks with a magnet.
The foam was channeled through several flasks with distilled water to collect the produced biosurfactant.
Precultures were prepared in conical shake flasks with initial OD600 of 0.5 and 120 rpm.
The batch equilibrium experiments were made in Pyrex 125 mL glass conical flasks with stoppers.
Strains were cultivated in shake flasks with YNB media and 2% glucose and FI was recorded.
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