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The flasks were kept on a magnetic stirrer at room temperature and the solutions changed to ruby red indicating the formation of gold nanoparticles.
The conical flasks were kept on a shaking incubator at ambient temperature (25 ± 2 °C) for 4 h to attain equilibrium.
These innoculated flasks were kept on a rotary shaker for two days and then the seed flasks were used to inoculate the remaining 30 flasks with spores of C. elegans which were again kept on shaker for incubation.
The flasks were kept on a rotary shaker (140 rpm) for 30 min and the extract was collected.
The flasks were kept on a shaker at 225 rpm in the dark and were sub-cultured every 5 weeks.
Then all the flasks were kept on a rotary shaker (140 rpm) for 15 min and then dried on sterile condition.
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Both flasks were kept on orbital shaker at 27 °C in 100 rpm under dark conditions.
The cell suspension cultures (30 ml each in 100 ml conical flasks) were kept on gyratory shakers (120 rpm) at 22±2°C under continuous light, and sub-cultured using 30 ml of 7-day cultures as inocula.
During the whole reaction time, the conical flask was kept on a shaker with constant speed at 130 rpm (rotations per minute).
Flasks were kept in a shaker incubator for 48 h (26 ± 1 °C, 130 rpm).
The flasks were kept in a shaking incubator at 25 °C with 100 rpm.
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