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Sixteen flasks of soil samples were placed in an electronic water bath at the same temperature as that at which the soil samples were incubated.
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The flasks were shaken for 20 min at 250 rpm, and aliquots (250 μL) of soil suspension were added to triplicate Erlenmeyer flasks containing 25 mL of MSM with 1% lignocellulose substrate (0.25 g in 25 mL), amended with a trace element and vitamin solution.
The quotient value of weight of the sample and its volume which was measured through volume of water displaced by known amount of soil sample in the volumetric flask is reported as particle density.
Ten grams of soil were collected from the flask to counter fungal population and DNA extraction at 5, 20, 30, 45, 60, 75 and 100 days after treatment (DAT).
1 g of soil sample was transferred to a flask containing 50 ml of sterile 0.85% physiological saline.
In this method, 10 g of soil was transferred into 250-ml Erlenmeyer flasks containing 100 ml of mineral salt medium (MSM).
To prevent anaerobiosis, the perforated hole of the flask lid was not sealed until 24 h before the measurement of soil organic carbon mineralization rate.
A 10 mL aliquot of each solution was transferred to amber glass flask and, immediately, added to the soil mass to a final concentration of 5 g L−1 of soil at OTC solution.
Fifty grams of soil and 100 mL of PO43− solution were added into each 250-mL Erlenmeyer flask.
You dropped two handfuls of soil.
For analysis, 350-400 mg (exactly weighted) of soil, roots or leaves were digested with 10 ml of concentrated HNO3 (65%) for 24 h at 130 °C in 25 ml round bottomed flasks equipped with reflux condensers.
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