Exact(2)
Cells were dissociated from flasks for analysis by means of 5 min incubation with 0.05% trypsin with 0.02% EDTA at 37°C.
ScFv-Fc-producing and growing cell pools were selected by enzyme-linked immunosorbent assay (ELISA) and expanded into T25 flasks for analysis of specific growth rates and specific productivities.
Similar(58)
MSC cultures were seeded into T75 culture flasks for protein analysis by western-blot, into 12-well plate for mRNA analysis (triplicate) and into 9 cm2 slideflask (Nunc) for immunocytochemistry.
Samples were taken daily from the first three flasks for ethanol analysis.
At a certain time interval, three flasks were fetched for analysis of DCW, lipid content, intracellular Monascus pigment concentration, and extracellular ones.
The aqueous phase sample was removed and put in a sealed flask for subsequent analysis.
We selected the small-scale flask-shaking fermentation method for analysis.
The sorted cells were either immediately used for analysis or placed in flasks for further Culturing.
At regular intervals of 48 h, 10 mL of culture broth was aseptically withdrawn from the flasks for growth measurements and residue analysis.
These cancer cells derived from subcutaneous xenograft tumors were cultured in flasks for expansion and second clonogenicity analysis.
For analysis of CO2 the cultures in flasks were acidified with HCl to lower the pH below 1.4.
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