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Cells were routinely grown in 75 cm tissue culture flasks and kept in a humidified atmosphere of 5% CO2 at 37°C.
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Extracted eggs and J2s were collected into a flask and kept in a refrigerator for 4 ± 1 days at 4 °C for further applications.
Equal mass of 0.1 g of the prepared gamma irradiated and steam-treated JSP was added to each flask and kept in an isothermal shaker at 30 °C.
Equal mass of 0.1 g of the prepared activated carbon with the particle size of 0.595 0.212 mm was added to each flask and kept in an isothermal shaker of 100 rpm at 25 °C to reach equilibrium.
The host insect cell line Sf9 (ECACC 89070101) was maintained in 50 mL working volume shake flasks (Corning, USA) and kept in a humidified incubator operated at 27°C and 90 rpm.
The reaction flask was then sealed tightly and kept in a water bath for polymerization.
The cryotubes were packed in a plastic bag and kept in a thermos flask containing water at 5-8°C 5-8°C
One millimolar of 95-ml silver nitrate (0.016 g) solution was prepared and kept in a 250-ml Erlenmeyer flask.
Briefly, 4.5 g of Dextran (Mr 15000−25000) and 0.63 g of FeCl3·6H2O were dissolved in 10 mL of cold, sterile water in a round-bottom flask purged with nitrogen and kept in an ice bath.
Magnetite suspensions were stored in anoxic Milli-Q (MQ) H2O in crimped-sealed serum flasks and kept in the dark.
For this purpose, 1 mL of the standard stock solution was taken in two different 25 mL volumetric flasks and kept in versatile environmental test chamber at 40°C/75% RH for 144 h and 288 h.
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