Sentence examples for flask were treated from inspiring English sources

The HB4a and HB4aC5.2 cell lines (10 cmlls/25 cm flask) were treated with 5 mL of medium containing 10% FBS, with or without DHA or trastuzumab.

The cell extracts from each flask were treated with Biotin alkyne (Click-it Biotin Protein Analysis Detection Kit, C33372, Invitrogen) and subjected to IP with SOCS1 antibody, followed by IB with Streptavidin-HRP.

The HB4a and HB4aC5.2 cell lines (10 cmlls/25 cm flask) were treated with 5 mL of medium containing 10% FBS, with DHA, trastuzumab, GW9662, or DHA plus trastuzumab, for 72 h.

Cells (1×10 cells/T-75 flask) were treated with 1 or 5 μM of the demethylating agent 5-aza-2′-deoxycytidine (5-Aza-dC) (Sigma-Aldrich) dissolved in 50% acetic acid or mock-treatment with PBS including the same amount of acetic acid every 24 hrs for 4 days.

Graphite (0.1 g; Sigma-Aldrich Co., St . Louis MO, USA) was put into a 25-mL round-bottomed flask, and the flask was treated using a CEM Discover Du7046 microwave synthesis system (CEM, Matthews, NC, USA) with a power output of 20 W at 80°C for 10 s.

The next day, each flask was treated with a different concentration of doxorubicin, decreasing in 3-fold increments, from 3.0 × 10-6 M to 5.13 × 10-11 M, with a final flask receiving no doxorubicin.

At transfer, a sample from the donor flask was treated with chloroform to stop phage growth, for plating at a later time; in one case of a phage with very low fitness, it was necessary to plate directly from the culture rather than from samples treated with chloroform (used with the initial T7αβ in M9).

SCAP cultured in a monolayer or cultured on NF-MS in spinner flasks were treated with 100 ng/ml BMP-2 in vitro.

Cells in 25-cm2 flasks were treated with 25 µM of erythrodiol, 50 µM of uvaol or EtOH for 6 or 18 h.

All glass coverslips and culture flasks were treated previously with a solution of 6.25 µg/ml cold poly-L-ornithine (0.16 ml/cm2 surface area) overnight.

Briefly, cells in 25-cm2 flasks were treated for 30 min with 1 µM DCFH-DA or DAF-FM and stimulated 30 min with 25 µM of erythrodiol, 50 µM of uvaol, or EtOH.