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Briefly, cells from a confluent tissue culture flask were spun at 1,000 rpm for five minutes and resuspended in 1.0 mL of growth medium.
Similar(59)
Cellular outgrowths were removed from the culture flask by scraping or aspiration with a pipette and were spun briefly to pellet the cells.
The parasites were spun down and washed three times in PBS before they were allowed to invade fresh HFFs in 75 cm2 flasks.
Our collective heads were spinning.
"People are spun up.
The centrifuge tube was spun down at 1000 rpm for 10 min and the resulting pellet gently resuspended in fresh media (10 mL) in a culture flask.
"The centrifuges are spinning".
Media heads are spinning!
"But it is spinning.
That's spin, of course.
They're spinning their wheels".
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