Sentence examples for flask were removed from inspiring English sources
When cells in primary culture reached 80%% confluence, cells not adherent to the culture flask were removed and the adherent astrocytes were dissociated from the flask surface using 0.25%% trypsin-1X EDTA.
Then, the conical flask was removed from the water bath and the hot reagent was decanted.
After a 2-h reaction at 180°C, the flask is removed from the heater and cooled at room temperature.
The aqueous layer at the bottom of the flask was removed using a syringe, and the toluene solution was concentrated by rotary evaporation at room temperature.
The resultant mixture then underwent microwave extraction, after which the flask was removed and the solution was water-cooled to room temperature.
Afterwards, the reacting solution in the flask was removed from the water bath and left stirring for 4 h at room temperature.
Refluxing (340°C to 360°C) was continued for either 30 or 60 min, and then the reaction flask was removed from the heat and allowed to cool to room temperature.
At the end of the desired contact time, the conical flask was removed from shaker and allowed to stand for 2 min for settling the adsorbent, and the adsorbent was filtered using Whatman No.42 filter paper.
The flask was removed from the rotary evaporator and stored in a desiccator at reduced pressure overnight to ensure complete evaporation of chloroform from the lipid cake.
At specific times, media in the flask was removed and placed in a sterile 15 mL conical tube and centrifuged (400 × G, 24°C for 10 minutes) to pellet the cells and the media was removed.
