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Postincubation, 3 samples of 2.5 × 10 cells from each flask were placed into 5 mL centrifuge tubes and spun at 300 g for 5 minutes.
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Each flask was placed in the P30 and treated.
The flask was placed in a shaker at 50°C and 200 rpm for 2 h.
The flask was placed under stirring at 150 rpm and 30 °C for 14 h.
A conical flask was placed at the bottom of the column to collect the eluted fractions.
The reaction flask was placed in an ice bucket to lower the reaction temperature to 0 °C.
The flask was placed on a magnetic hot plate with continuous stirring and then attached to the Schlenk line.
The flask was placed in a temperature-controlled water bath set at 70 °C for 90 min, under atmospheric pressure.
The air in the flask was replaced by nitrogen, and then the flask was placed in a constant temperature oil bath at desired temperature.
The flask was placed in a preheated oil bath maintained at 70°C, and the FTIR spectra were collected at the time.
The flask was placed in an oil bath and the desired temperature of esterification reaction was achieved and the catalyst PTSA was loaded.
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