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To a 50 ml round bottom flask were added p-hydroxyl benzoic acid 1 (10 g, 72.5 mmol) and acetic anhydride (15 ml).
Into a 100 mL round bottom flask were added 250 mg (1.00 mmol) of 6,7-dichloro-2-methylquinoline-5,8-dione (1), ethanol (30 mL), CeCl3·7H2O, and alkoxy substituted aryl amines (2), respectively.
Secondary alloreactive cultures were prepared similarly in RP-10 media except that 2.5 × 10 1° effectors per flask were added together with 25 × 10 irradiated BALB/c spleen cells to upright T-25 flasks.
For 1° alloreactive stimulation, 25 × 10 B6 spleen cells per flask were added to upright T-25 flasks with 25 × 10 BALB/c irradiated spleen cells in 10 mL RP-10 media.
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The flask was added with 10.00 mL of 3, 5-dimethylphenyl isocyanate (2), then the solution was refluxed for 48 h.
Then, a flask was added 210-mg polyvinylpyrrolidone (K 30, average Mw 40,000, Sigma Aldrich) and 100 mL of 3.876 mM Na2PdCl4.
To a flam-dried 250 mL 4-necked flask, was added 7.92 g (24.4 mmol) of compound 3 and 18.6 mL TFA.
In detail, 50 ml deionized water in a round-bottom flask was added to 5, 10, 20, 30, 40, and 50 mg chloroauric acid, respectively.
As shown in Figure 1, 1.00 g of dry microcrystalline cellulose was transferred into a 100 ml three-neck flask, and the flask was added with 34.00 g of AmimCl (1).
To a cooled t-3, t-5-dimethyl-r-2,c-6-diphenylpiperidin-4-one (1) [1.40 g, 5 mmol] solution in ether (20 30 ml) in an Erlenmeyer flask, was added conc.HCl until precipitation of the white solid was completed (5 10 min).
To a four-necked round bottom of flask, was added 0.6 g (2.17 mmol) of compound 6 and 10 mL 1,4-dioxane, then 3.3 g of polyformaldehyde and 4 drops of TEA.
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