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Subsequently, the flask was transferred into a water bath set at a certain temperature.
The desired amount of AuNPs-PSNPs was added and reaction flask was transferred to ultrasonic bath cleaner for 6 h.
After 24 h of growth, the entire broth from the growth flask was transferred to a conversion flask.
The flask was transferred to a preheated oil bath (270 °C) and heated for 6 h with mechanical stirring under nitrogen atmosphere.
An aliquot (100 μL) of the content of each Erlenmeyer flask was transferred to a microtube filled with 900 μL of sterile NaCl aqueous solution.
Seed culture (300 ml), cultivated at 30°C for 17 h in shake flask was transferred to the fermenter containing 2.7 L of the fermentation medium to initial production in bioreactor.
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Three aliquots of 200 μl from each flask were transferred to a sterile Costar 96-well black plate with flat clear bottom (Corning).
Subsequently 1 mL of the inoculum having approximately 5 × 103 CFU/mL cell density from the flasks was transferred into sterile medium (100 mL) containing supplementation of same lindane concentration.
After 2 h of culturing, cultures from the three shake flasks were transferred into the fermenter.
When 16°C had been attained, flasks were transferred to a 16°C water bath while constantly stirring.
Shake flasks were transferred to 20°C shakers at an OD600 of 0.2, and L-cysteine was added to 1 mM.
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