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The mycelia formation in the flask was observed periodically.
Fourth, the yield of 12% achieved in shake flask was observed during the post-induction, linear isopentenol production period.
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In contrast, evaporation of two-thirds of the starting volume of liquid CeMM in 75 cm2 flasks is observed to take four to five weeks.
Individual culture flasks were observed daily and photographed by a phase-contrast microscope for the presence of cell monolayers and morphologies.
For growth properties and morphology study in vitro, population doubling times and cell viability were determined and cells grown on 75-cm culture flasks were observed daily by phase-contrast microscopy (Park et al, 1995).
One drop of antifoaming agent, antifoam B emulsion, was dispensed in each flask after initial foaming was observed during aeration.
After 12 days of culturing in the feather meal medium, complete decomposition of feathers was observed only in flask B4.
Consistent with this hypothesis, a 22 and 27% increase of HA yield was observed with the flask cultures of ΔfruA and ΔfruK, respectively (Fig. 5c).
On flask surfaces, mineralized bone nodules formation was observed and stained with Alizarin red (Fig. 2D).
After 8 10 days of culture, the first colony was observed in each flask.
No pellicle formation was observed in the influent flask during the 72 h experiment.
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