To 0.5 g grounded plant material, 10 mL of diacidic mixture was added and the contents of the flask were mixed by swirling.
For all blood culture systems, the blood culture flasks were mixed with 10 ml of blood and then incubated at 37°C for 7 days.
For cell counting, 1 ml of fresh sample (one from the supernatant and one from the flask surface) was mixed in a BD Trucount Tube (Becton Dickinson), with a known number of beads, so absolute cell number counts could be calculated, using an LSR Fortessa flow cytometer (Becton Dickinson).
Then in the flask, propolis was mixed with 80%% ethanol at a ratio of 1 g: 10 mL and mixtures were shaken (Certomat Model S II, Sartorius, Goettingen, Germany) at 200 rpm for 24 h at 60 °C and centrifuged (Eppendorf Model 5810 R, Hamburg, Germany) at 3000 rpm for 15 min at 10 °C.
To 1 ml of this solution 45 ml of distilled water and 1 ml of Folin-Ciocalteu Reagent (FCR) was mixed in a volumetric flask.
At the beginning of each experimental run, 50 ml of phenol solution was mixed with the adsorbent in a conical flask and the flasks were agitated on a shaking water bath at the required temperature.
In brief, a solution of POPC/POPG (80/20 molar ratio) dissolved in chloroform was mixed in a round bottom flask, after which the solvent was completely evaporated.
Briefly, 5 mg of different ratios of MePEG and ε-CL was mixed in a 10-ml round bottom flask connected to vacuum.
An aqueous solution of sodium tris-citrate (5 mL, 1%) was mixed with HAuCl4(1 mL, 1%) solution in a conical flask and sealed with parafilm.
