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Each flask was contained 150 mL of distilled water and then autoclaved at 121°C for 30 min [ 18].
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When solutions are evaluated to sorb mercury, the system is assembled as shown in Figure 1, and a 150-mL glass flask is used, containing 50 mL of the solution to be tested, in EHg position.
Growth of Lemna aequinocitalis in compound containing flask was determined by counting the number of fronds per dose and growth inhibition calculated with reference to negative control.
From weeks 2 to 7, when activity of zoospores was no longer visible and growth appeared to have ceased, 0.5 mL of water from each flask was added into flasks containing TGhL media and incubated at 23°C.
Seed culture (300 ml), cultivated at 30°C for 17 h in shake flask was transferred to the fermenter containing 2.7 L of the fermentation medium to initial production in bioreactor.
The culture medium in each flask was then replaced with medium containing 0.5% FBS.
The culture medium of each flask was then replaced with medium containing 0.5% FBS.
The Büchner flask was then connected to the flask containing degassed PBS.
In the anaerobic cultivations, each flask was equipped with a loop trap containing sterile glycerol.
For the repeated batch fermentations, conical flasks were used containing immobilized mixed anaerobic culture on 100 g cylindrical γ-alumina and 100 mL of synthetic medium containing 20 g/L sugars.
C N ratio utilization: For determining the C N ratio, 250 mL shake flasks were used containing dextrose as the carbon source and peptone as the nitrogen source.
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