The reagent preparation will generate a lot of acid fumes, thus the flask top must be firmly set and clamped in place (or it will pop up during the boiling and spit hot acid around) and a water trap, ideally a gas wash bottle, should be connected to the outlet of the flask (Figure 1).
The following day flasks were topped up with osteoinductive medium, (basal medium supplemented with 0.25 mM ascorbic acid, 10 mM β-glycerophosphate and 100 nM dexamethasone (all Sigma-Aldrich)).
After the supernatants were pooled, transferred to a 25-mL volumetric flask and the volume was topped up with the same solvent.
And then there is the suave Ship's Decanter, a flask flared toward the bottom, with a narrow neck adorned by four glass rings and topped by a pierced round stopper.
A single bottle of cheap chardonnay doesn't reach the flask's fill line, so I top it up with a different chardonnay.
Just before we come upon the first boss in Dark Souls III (discounting that crystal dragon beast), we pause at what Serafinowicz affectionately calls a "bonnie," a bonfire, to top up our health-restoring Estus Flasks.
Just before we come upon the first boss in Dark Souls III (discounting that crystal dragon beast), we pause at what Peter affectionately calls a "bonnie", a bonfire, to top up our health-restoring Estus Flasks.
Rinse flask with clear water.
The round-bottom flask was mounted on top of a magnetic stirrer, and the reaction was carried out with continuous stirring.
One of the most magical nights I ever had in St. John's was passing around a flask of Old Sam on top of the Supreme Court while we were blitzed out of our minds on hash.
There were also sumptuous wine coolers, cake and pudding molds, bowls, buckets, jugs, jars, screw-top flasks, sausage pans, and many other items, all polished until they shone and thus used as kitchen decorations as well as utility items.
