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The reagent preparation generates a lot of acid fumes, therefore the flask top must be firmly set and clamped in place and a gas wash flask attached to the outlet.
The reagent preparation will generate a lot of acid fumes, thus the flask top must be firmly set and clamped in place (or it will pop up during the boiling and spit hot acid around) and a water trap, ideally a gas wash bottle, should be connected to the outlet of the flask (Figure 1).
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Solution was transferred into a 1-L Erlenmeyer flask and topped up with deionized water until it reached 575mL and refluxed under heating for 4 h.
The following day flasks were topped up with osteoinductive medium, (basal medium supplemented with 0.25 mM ascorbic acid, 10 mM β-glycerophosphate and 100 nM dexamethasone (all Sigma-Aldrich)).
Wealth of Nations handcrafted to include a hidden 250 ml swing top glass flask imported from Italy.
The round-bottom flask was mounted on top of a magnetic stirrer, and the reaction was carried out with continuous stirring.
One of the most magical nights I ever had in St. John's was passing around a flask of Old Sam on top of the Supreme Court while we were blitzed out of our minds on hash.
H2SO4 was attached to the top of each flask to trap water evaporated from the flask during the fermentation.
Chemical reagents, syringes, Petri dishes, pipette tips and Erlenmeyer flasks sat on the top shelf, next to a pile of T-shirts.
PFA stock solutions may be frozen until use, but should be kept in capped flasks filled to the top and sealed with parafilm to avoid oxidation.
Ticks were placed in 75-cm tissue culture flasks pierced at the top to accommodate a 4-cm diameter glass feeder.
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