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Flask fermentation experiments also confirmed that two-step route can significantly improve the sabinene titer to 150 mg/L (6.5-fold of the sabinene titer in an earlier flask study of our lab).
Our finding from the shake flask study showed that oxygen limitation has increased the accumulation of PHA by Comamonas sp. EB172 (unpublished data).
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Interestingly, only 0.17 mol% 4HB in the copolymer was obtained during shake flask studies.
The feed rate adjustments were done according to observations in the previous shake flask studies.
Accordingly, combinatory gene-knockout strains were constructed and characterized, through which the molar fraction of 4HB was increased by 24-fold in shake flask studies.
Shake flask studies showed that the 3HV fraction in the copolymers increased approximately 16-folds, demonstrating enhanced effectiveness of the ΔprpC mutant to synthesize PHBV.
Whereas in flask studies, sulfate ion alone enhanced TCE (at 6 ppm) degradation rates over those found in the absence of suitable additives, benzoic acid showed enhanced degradation rates for TCE at 6 50 ppm levels.
In shake flask studies, the cell density and 2-KGA production of the recombinant K. vulgare Rif (pMCS2PsdhfolBC) were increased by 18% (P < 0.001) and 14% (P < 0.001), respectively, under a relatively stable pH 7 condition.
The above E. coli recombinants grown on glucose LB medium successfully produced 5 g/L cell dry weight containing 18% P3HP and 42% P 3HB-co-84 mol% 3HP 3HB-co-84ively, in 48 h shake flask studies.
Interestingly, shake flask studies indicated that PTE1 harboring strain showed advantages over tesB expressing one for 3HDD and 3HD production, while tesB favored 3HTD production by P. entomophila LAC31.
Batch shake flask studies were performed firstly by conducting fermentations at the temperatures 30°C or 37°C.
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