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Here we note that g and Tmax were separately estimated and fixed to be 0.47 ± 0.10 for the static culture and 0.54 ± 0.09 for the shaking culture per day, and (1.51 ± 0.02) × 10 and (1.22 ± 0.02) × 10 cells per flask of medium from the cell growth experiments, respectively (see 'Materials and methods', Figure 2 figure supplement 2 and Supplementary file 2).
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These flasks of medium were allowed to equilibrate at 37 °C in a humidified 95%air/5%% CO2 atmosphere for at least four days before the pH was determined.
The cultures were incubated at 150 rpm on rotary shaker at 30 °C for 4 days in 50 mL Erlenmeyer flasks with 10%% of medium in each flask, and a flask without cell mass was used as controls.
The CYP3A4 level was about three times higher than that obtained by incubation in a 500 mL flask (100 mL of medium) (84 nmol/L culture).
A stock culture of B. braunii SAG807-1 was cultured in a 1 L Erlenmeyer flask containing 0.7 L of medium.
The selected flask is drained of medium, inoculated with 1 to 2 ml of the infected fluid harvest and held stationary for 30 minutes at 37°C.
Individual transformant clones were grown overnight at 37°C in 10 ml of Luria-Bertani (LB) medium containing 50 µg ml−1 carbenicillin and 34 µg ml−1 chloroamphenicol, and used to seed 500 ml flasks of LB medium supplemented with the appropriate antibiotics.
In brief, conidia from agar media were used to inoculate flasks of liquid medium (40 g L-1 glucose, 20 g L-1 yeast extract, 15 ml L-1 corn steep liquor).
In cultures for production of S-HMGSH dehydrogenase, 3-L shaking flasks containing 1 L of medium were inoculated with 1.5% (v/v) seed culture and cultured on a reciprocal shaker (120 strokes/min) at 25°C for 1 week.
A single bacterial cell innoculated in a large flask of constantly stirred medium will divide into two cells after about an hour.
About of 1 ml of inoculum was inoculated into 19 ml/100 ml flask of liquid minimal medium, and the flasks were incubated at 37 °C at 100 rpm for 120 h.
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