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On March 18, researchers noticed that something was happening in a flask of cells that had been seeded with a sample from the throat of a patient.
A batch of cells is a single flask of cells that has undergone the entire differentiation protocol and is considered a single biological replicate.
Kevin Wang holds a flask of cells that have been engineered to produce the Regina protein, a mammalian protein similar in structure to the active component of honeybee royal jelly.
One flask of cells was reserved as 0-hours baseline control.
A healthy confluent flask of cells was trypsinised using 0.25% w v−1 trypsin solution and 1 m M versene solution in a 1 : 1 mixture.
This phage-cell mix was grown long enough to achieve a phage density allowing transfer of at least 105 phage into a new flask of cells.
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Eight flasks of primary human renal tubular epithelial cells (Cambrex, Walkersville, MD) were exposed to medium alone and 8 flasks of cells were exposed to medium containing 1% bovine serum albumin for 6 h.
Separate flasks of cells were used for each of the treatments and assays.
Cell-conditioned medium was aspirated from near confluent flasks of cells and stored at -80 °C, for subsequent perlecan purification.
It suggests that the protocol would benefit from pooling three or more transfected flasks of cells prior to sorting.
Each experiment was repeated 2 to 3 times with duplicate flasks of cells (n number presented in the graphs).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com