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Each mouse was then placed on the conical flask in order to obtain its response to heat-induced nociceptive pain stimulus.
The reaction assay was prepared in the dissolution flask in order to mimic the same release pattern of the tablets in the dissolution experiment.
The solution was transferred into a large round-bottom flask in order to avoid loss of product caused by foam, and evaporated to dryness, rinsed with water and evaporated, and the procedure repeated until an ammonia odourless solution was obtained.
The use of a mini-fermentor could be considered for scale-up experiments instead of culture in a shaken flask in order to improve the efficiency of product production and the level of expression of foreign protein in P. pastoris.
Our approach was to remove one or more of the defined components of SYN6.4 medium in each flask in order to discover "inessential" constituents and those that are limiting for rSAK-2 expression.
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Also, agarose concentration was kept at 1% in all flasks in order not to influence possible differences between concentrations.
Metal and metal oxide NPs suspensions were added to the same algal culture (approx. 106 cells/mL) in 15-mL glass flasks in order to achieve final concentrations of 1, 5, 10 and 20 mg/L.
Both the M14K adherent line cells) and the K562 suspension line cells) proliferated in 250 mL flasks in order to obtain a sufficient number of cells (4 × 10 cells for M14K and 1 × 10 cells for K562).
Cell expansion was conducted in 250 mL flasks in order to get a sufficient number of cells (4 × 10 cells for M14K and 1 × 10 cells for the K562 line).
A calculation of the carbon recovery (CR), on c-mole basis, was done for both the hybrid SSCF and shake flask experiments in order to see if all major products had been accounted for.
Precultures in ODM with 150 g/L glucose were incubated as described above for 96 h prior to use as inocula for the test flask cultures in order to produce large populations requiring enzyme induction for xylose utilization.
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