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Finally, the GlcNAc titer was further increased to 31.65 g/L, which was 3.8-fold that in the shake flask, in a 3-L fed-batch bioreactor.
Not really, but she replies she is thirsty, to be polite, and he raises a flask in a camouflage cover, and she sips, her lip touching where his lip has been.
These we can make in large numbers, in flask, in a lab and we've shown they are as good as the best single crystals," says co-lead author David Hanifi, a graduate student in chemistry at Stanford University.
Antibody-producing cells that would be housed in a glass flask in a research laboratory are grown in a stainless steel tank nearly the size of a fuel tank on a semi truck.
Out came a crystal hip flask in a silver jacket with A'Lelia Walker's monogram, and a square decanter, its lid inscribed with her name, its spout cleverly secured with a lock to protect it from the constant parade of guests at the Walkers' homes.
These we can make in large numbers, in flask, in a lab and we've shown they are as good as the best single crystals," said David Hanifi, graduate student in chemistry at Stanford and co-lead author of the paper written about this work, published March 15 in Science, DOI: 10.1126/science.aat3803.
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Before the experiment, the pathogens were cultured in Mueller-Hinton broth in a flask in an orbital shaker at 37°C.
Cells were grown in 50 mL of buffered complex medium in a 250-mL flask in an orbital shaker (250 rpm) at 28 °C to an OD600 of 3 6.
Synoviocytes were then trypsinized and grown in 75 cellculturelture flasks in a humidified atmosphere (5% v/v CO2) at 37 °C.
Ionic liquid was added to the flasks in a 1 4 mass ratio.
Six 40-W straight fluorescent tubes (Phillips) were placed 20 cm above the flasks in a dark room.
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