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Subsequently, Escherichia coli cell-killing experiments were performed in both shake flask (i. e. batch-mode) and flow-column (i. e. continuous-mode) operations, using E. coli K12 (MTCC 1302) as a model organism.
Though frugal travel has required me to embrace certain Buddhist conventions — shedding attachments to luxuries, for example — the closest I'd ever come to spiritual enlightenment was drinking bourbon from a silver Tibetan flask I bought in India.
The lyrics, apparently about a drug dealer whose girlfriend is giving him a hard time about his occupation, defends his ill-gotten gain: "Long as I'm nice with the flame and the flask, I don't mind keeping you up on the must-haves.
10/15 Frank Turner chose 'By The Lamplight' by Larry and His Flask I spent the end of last year and the start of this touring with this band around the release of this album but I genuinely think it's a thing of wonder.
"Here I have my own lab and do my own research, and no one tells me who to hire or what kind of tissue culture flask I have to buy".
Can I fill up the flask I know you have on you somewhere?" Sure, they all have a different way of saying it.
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To stopper my flasks, I got some tight-fitting rubber bungs and corks, some with holes in the middle for glass tubes.
However, the TP concentrations in the MX and MX-C flasks (i.e. with added NaNO3) were relatively low at the end of the cultivation period (see Table 2), especially in the MX group where the TP concentration was found to correspond to the lowest value measured: 4.71 mg L−1.
Subsequently, approximately 30 Mn II1 Mn(II) was added to all of the flasks (i.e., with or without proteinase K), which were maintained at 37°C and 150 min−1 for three days.
"My next plan is to get a pan and a Thermos flask so I can sell coffees and teas," he says.
I was at home over the Bank Holiday weekend so clambered up into the loft to root through all my old university junk, retrieving a couple of pieces of tupperware and my old vacuum flask that I used to take everywhere.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com