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One flask from each set was removed from the shaker every four hours.
The media for one daughter flask from each cell line was supplemented with rhDcR3-Fc at a concentration of 0.1 μg/ml.
One flask from each subject was left to grow in normal glucose (2%) while the other flask of cells was transferred into complete media containing 5.55 mM d-glucose for 24 hours.
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Finally transfection complexes were added dropwise to two culture flasks from each Module.
Upon reaching near-confluence, cell culture medium was removed, cells were washed with PBS, and 1 ml of culture medium was added back to each of three flasks from each cell line.
At this point, individual culture flasks from each horse were exposed to Gey's Balanced Salt Solution (unchallenged control), 100 ng/ml LPS, or 1,000 ng/ml LPS for 2 hours.
For fungal inoculum propagation, each flask from the above step was transferred to 10 L capacity Erlenmeyer flask contained 1 kg sterilized moistened SBP, and then incubated statically at 32 °C for 4 days.
Flasks, one from each donor, were harvested and assayed on D1, D3, D5 and D7.
Bartender produced flask from coat.
One took a plastic flask from an incubator and placed it under a microscope.
She took a flask from her bag and opened it, releasing the pungent smell of bootleg whiskey.
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