The first flask consisted of formation water without inhibitor, and the second consisted of formation water with 30 mg/L scale inhibitor.
The hydrolysis experiment was conducted in 100-mL Erlenmeyer flasks consisting of 1.0 g residue from CS after the H2O2-LiP hydrolysis, 0.1 mL liquid cellulase, and 20 mL distilled water at pH 6.0.
SSF process for laccase production by Trichoderma harzianum strain HZN10 was performed in 250-ml glass (Erlenmeyer) flasks consisting 10 g of each material (WB, RS, SB, CC, SD, GS and LL) individually moistened (70%) with the liquid media containing (g/L) (NH4 2SO4 1, CaCl2 0.125, NaH2PO4·H2O 1 and MgSO4·7H2O 0.5 without glucose (Bagewadi et al. 2017).
About 35% of the total initial number of BMSCs obtained by a fractionated isolation protocol based on the time of cell adhesion to the culture flask surface consisted of cells that required longer to adhere.
In brief, confluent p2 MEF were irradiated (5000 cGy) and seeded at 6×104 cells cm−2 on gelatin coated flasks in MEF medium consisting of DMEM (with Glutamine), 10% fetal bovine serum (FBS, Characterized, Hyclone, Thermo Fisher, Waltham MA, http://www.hyclone.com), 1% NEAA, 55 µM 2-mercaptoethanol.
Vero E6 cells (American Type Culture Collection, Manassas, VA) were propagated in 75 cm cell culture flasks in growth medium consisting of medium 199 (Sigma, St Louis, MO) supplemented with 10% fetal calf serum (FCS; Biological Industries, Kibbutz Beit Haemek, Israel).
HCT-C18 (TS-) cells were maintained in 75-cm2 plastic tissue culture flasks with growth medium consisting of RPMI 1640 medium containing 10% fetal bovine serum and supplemented with 10 μM thymidine (Sigma, MO) at 37°C and 5% CO2.
A colony of the strain ZX09 was inoculated into a 250 ml flask containing 50 ml medium consisting of 2%% sucrose and mineral salt solution.
When fermentation was complete, all ethanol was distilled from worts using a laboratory distillation unit consisting of a distillation flask, a Liebig cooler, a flask for collecting ethanol, and a thermometer.
