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To our knowledge, this is the first study to report that the CO2 concentration increases (~85.0 mg/L) during shake-flask culture using a culture plug-capped Erlenmeyer flask, compared with the initial culture condition (~4.0 mg/L).
In general, T-MPCs were somewhat smaller than BM-MPCs, with a cell yield of 7 to 10 × 106 cells per 80% confluent Nunc triple flask compared with 4 to 8 × 106 BM-MPCs.
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Although a low embryo-to-culture volume ratio is critical for the optimal development of embryos in vitro [54] [56]it would be impossible to produce sufficient numbers of embryos to compensate for the 1000-fold greater volume of flasks compared with microdroplets in dishes.
As a result, an optimal factor-shift process was determined and the soluble yield of the target protein increased by 82.9% (from 68.9 to 126.6 mg protein/g DCW) in flask fermentation compared with the result cultured at 30 °C.
At 96 h after IVF, the rate of blastocyst development was significantly decreased in flask cultures compared with routine dish cultures (63% vs 97%, respectively; repeated three times).
The results indicated that the yield of modified protein could be increased by up 3-fold in bioreactor as compared with flask.
Compared with flask-cultured cells, RCCS culture induced an accumulation of cell number at S phase concomitant with a decrease at G0/G1 and G2/M phases at 12 h.
The reaction kinetics of G1 dendron synthetic process was conducted using the microreactor and compared with the flask synthesis.
Compared with shake flask, bioreactor offers a relatively stable and controllable extracellular environment for cell growth and metabolism.
It is thus reasonable to speculate that the yield of these two enzymes could significantly increase under fermentor culturing conditions, based on literature reports showing that the expression of Tr EGI in Y. lipolytica in fed-batch fermentation combined with high-cell density cultivation techniques has been increased 20-fold compared with the flask culture [ 20].
Given the differences in physical characteristics between the ambr™ and larger stirred bioreactors, we suggest that this similarity in biological performance is due to their similar control capabilities and the 'equivalence of the stress parameters' across the scales when compared with shake flasks.
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