Sentence examples for flask base from inspiring English sources
The plastic flasks were then cracked open using pliers and the explant culture, or cell line clones were processed on the flask base as for specimens mounted on glass slides.
Organotypic biopsy spheroids from human GBMs were prepared as previously described [ 4, 27] Briefly, tumor samples were minced into ~0.2 mm fragments and placed into tissue culture flasks base coated with 0.75 % agar (Difco, Detroit, MI, USA).
Growth rates were calculated in shake flask cultures based on the difference in the log absorbance per unit time at mid-exponential growth phase when the slope of the growth curve was maximum.
In this work, the solubility of -catechin in water and water-ethanol mixtures was measured spectrophotometrically using a novel shake-flask method (based on a Carousel 12 Plus™ Reaction Station) within the temperature range 277.6 331.2 K.
The mass transfer into the film on the flask wall and base at "in-phase" operating conditions is described by Higbie's penetration theory.
A spatially- and time-resolved consideration of the mass transfer in the liquid film on the flask wall and base has shown that the validity of Higbie's theory sensitively depends on the film thickness and contact time.
Finally, a sample of soil suspension and duplicate flask samples (selected based on reported DGGE analyses) at the final batches were taken from the RWS and TWS consortia after 1 (T1), 3 (T3) and 10 (transferssfers (n = 13) and used for total DNA extractions and pyrosequencing as described below.
A second series of shake-flask experiments was based on autoclaved non-supplemented DW medium.
The aim of this study was to develop an efficient cultivation process based on shake flask experiments.
Based on the flask test results, a 1/3 of the design matrix was chosen for the bioreactor experiments.
Cells were removed from the base of the flasks using a cell scrapper, pooled and placed in 400 ml glass bottles and stored at −80 °C prior to the virus purification step.
